a431 parental cells Search Results


parental a431 cells  (ATCC)
98
ATCC parental a431 cells
Fig. 1 – Expression levels of RPS12 and c-Myc were analysed in <t>A431-III</t> and A431-P cells. A. Western blot analyses of RPS12 and c-Myc expressions were conducted using specific antibodies. The multiples of change on the western blots shown in (B) were analysed using ImageJ software.
Parental A431 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a431+parental+cells/A-431/10__1016_slash_j__jff__2015__09__030-58-0-15
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parental a431 cells - by Bioz Stars, 2026-10
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cytokeratin 17 antibody  (NSJ Bioreagents)
99
NSJ Bioreagents cytokeratin 17 antibody
Fig. 1 – Expression levels of RPS12 and c-Myc were analysed in <t>A431-III</t> and A431-P cells. A. Western blot analyses of RPS12 and c-Myc expressions were conducted using specific antibodies. The multiples of change on the western blots shown in (B) were analysed using ImageJ software.
Cytokeratin 17 Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/a431+parental+cells/Cytokeratin+17+Antibody/custom%40v2176%4010574702
Average 99 stars, based on 1 article reviews
cytokeratin 17 antibody - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier

Image Search Results


Fig. 1 – Expression levels of RPS12 and c-Myc were analysed in A431-III and A431-P cells. A. Western blot analyses of RPS12 and c-Myc expressions were conducted using specific antibodies. The multiples of change on the western blots shown in (B) were analysed using ImageJ software.

Journal: Journal of Functional Foods

Article Title: RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

doi: 10.1016/j.jff.2015.09.030

Figure Lengend Snippet: Fig. 1 – Expression levels of RPS12 and c-Myc were analysed in A431-III and A431-P cells. A. Western blot analyses of RPS12 and c-Myc expressions were conducted using specific antibodies. The multiples of change on the western blots shown in (B) were analysed using ImageJ software.

Article Snippet: Parental A431 cells (human epidermal carcinoma cells called A431-P in this study) were obtained from ATCC (Manassas, VA, USA).

Techniques: Expressing, Western Blot, Software

Fig. 2 – Cell viability analysis of A431-III cells treated with flavonoids and inhibitors using an MTT assay. The structures of luteolin and quercetin are shown in A and B. A431-III cell viability was analysed after treatment with different concentrations of luteolin (Lu) and quercetin (Qu) (A), rapamycin (C), LY294002 (D), and 10058-F4 (E) for 24 h via the MTT assay.

Journal: Journal of Functional Foods

Article Title: RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

doi: 10.1016/j.jff.2015.09.030

Figure Lengend Snippet: Fig. 2 – Cell viability analysis of A431-III cells treated with flavonoids and inhibitors using an MTT assay. The structures of luteolin and quercetin are shown in A and B. A431-III cell viability was analysed after treatment with different concentrations of luteolin (Lu) and quercetin (Qu) (A), rapamycin (C), LY294002 (D), and 10058-F4 (E) for 24 h via the MTT assay.

Article Snippet: Parental A431 cells (human epidermal carcinoma cells called A431-P in this study) were obtained from ATCC (Manassas, VA, USA).

Techniques: MTT Assay

Fig. 3 – c-Myc expression is inhibited by luteolin and quercetin through Akt/mTOR signalling. A. A431-III cells were treated with control (DMSO), luteolin (10 µM, 20 µM), and quercetin (20 µM, 40 µM) for 24 h. Cell lysates of A431-P cells and A431-III cells treated with different concentrations of luteolin and quercetin were collected and analysed by western blotting using specific antibodies. B. A431-III cells were treated with DMSO, 5 or 10 nM rapamycin, or 5 or 10 µM LY294002 for 24 h. Cell lysates of A431-P cells and A431-III cells were treated with different concentration of rapamycin or LY294002 were collected, and the expressions were detected by western blotting using specific antibodies.

Journal: Journal of Functional Foods

Article Title: RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

doi: 10.1016/j.jff.2015.09.030

Figure Lengend Snippet: Fig. 3 – c-Myc expression is inhibited by luteolin and quercetin through Akt/mTOR signalling. A. A431-III cells were treated with control (DMSO), luteolin (10 µM, 20 µM), and quercetin (20 µM, 40 µM) for 24 h. Cell lysates of A431-P cells and A431-III cells treated with different concentrations of luteolin and quercetin were collected and analysed by western blotting using specific antibodies. B. A431-III cells were treated with DMSO, 5 or 10 nM rapamycin, or 5 or 10 µM LY294002 for 24 h. Cell lysates of A431-P cells and A431-III cells were treated with different concentration of rapamycin or LY294002 were collected, and the expressions were detected by western blotting using specific antibodies.

Article Snippet: Parental A431 cells (human epidermal carcinoma cells called A431-P in this study) were obtained from ATCC (Manassas, VA, USA).

Techniques: Expressing, Control, Western Blot, Concentration Assay

Fig. 4 – Ribosomal protein S12 (RPS12) expression is inhibited by luteolin and quercetin through Akt/mTOR signalling. A. A431-III cells were treated with DMSO, 10 or 20 µM luteolin, or 20 or 40 µM quercetin for 24 h. Cell lysates of A431-P cells and A431-III cells were collected and analysed by western blotting using an RPS12 antibody. B. Changes in RPS12 expression after treatment with luteolin and quercetin were analysed using ImageJ software. C. A431-III cells were treated with DMSO, 5, 10 nM rapamycin, or 5, 10 µM LY294002 for 24 h. Cell lysates of A431-P and A431-III cells were collected and analysed by western blotting using an RPS12 antibody. D. Changes in RPS12 expression after treatment with rapamycin and LY294002 were analysed using ImageJ software.

Journal: Journal of Functional Foods

Article Title: RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

doi: 10.1016/j.jff.2015.09.030

Figure Lengend Snippet: Fig. 4 – Ribosomal protein S12 (RPS12) expression is inhibited by luteolin and quercetin through Akt/mTOR signalling. A. A431-III cells were treated with DMSO, 10 or 20 µM luteolin, or 20 or 40 µM quercetin for 24 h. Cell lysates of A431-P cells and A431-III cells were collected and analysed by western blotting using an RPS12 antibody. B. Changes in RPS12 expression after treatment with luteolin and quercetin were analysed using ImageJ software. C. A431-III cells were treated with DMSO, 5, 10 nM rapamycin, or 5, 10 µM LY294002 for 24 h. Cell lysates of A431-P and A431-III cells were collected and analysed by western blotting using an RPS12 antibody. D. Changes in RPS12 expression after treatment with rapamycin and LY294002 were analysed using ImageJ software.

Article Snippet: Parental A431 cells (human epidermal carcinoma cells called A431-P in this study) were obtained from ATCC (Manassas, VA, USA).

Techniques: Expressing, Western Blot, Software

Fig. 5 – c-Myc regulates ribosomal protein S12 (RPS12) expression. A. A431-III cells were treated with DMSO, or 100, 200, and 400 µM 10058-F4 for 24 h. Cell lysates of A431-P and A431-III cells were collected and analysed by western blotting using a RPS12 antibody. B. Changes in RPS12 expression after treatment with 10058-F3 were analysed using ImageJ software. C. The pcDNA3-cMyc-HA plasmid at 0.5, 1, or 1.5 µg was transfected into A431-P cells for 48 h. Cell lysates were collected and analysed by western blotting using c-Myc and RPS12 antibodies. D. Changes in RPS12 expression after transfection with the pcDNA3-cMyc-HA plasmid were analysed using ImageJ software.

Journal: Journal of Functional Foods

Article Title: RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

doi: 10.1016/j.jff.2015.09.030

Figure Lengend Snippet: Fig. 5 – c-Myc regulates ribosomal protein S12 (RPS12) expression. A. A431-III cells were treated with DMSO, or 100, 200, and 400 µM 10058-F4 for 24 h. Cell lysates of A431-P and A431-III cells were collected and analysed by western blotting using a RPS12 antibody. B. Changes in RPS12 expression after treatment with 10058-F3 were analysed using ImageJ software. C. The pcDNA3-cMyc-HA plasmid at 0.5, 1, or 1.5 µg was transfected into A431-P cells for 48 h. Cell lysates were collected and analysed by western blotting using c-Myc and RPS12 antibodies. D. Changes in RPS12 expression after transfection with the pcDNA3-cMyc-HA plasmid were analysed using ImageJ software.

Article Snippet: Parental A431 cells (human epidermal carcinoma cells called A431-P in this study) were obtained from ATCC (Manassas, VA, USA).

Techniques: Expressing, Western Blot, Software, Plasmid Preparation, Transfection

Fig. 6 – The transactivation of ribosomal protein S12 (RPS12) is regulated by Akt/mTOR/c-Myc signalling. A. The pGL3-basic plasmid at 0.5 µg, or 0.5 or 1.0 µg of the pGL3-RPS12-pro plasmid was transfected into A431-P cells for 48 h, and the transactivation activities were analysed using a luciferase assay. B. The pGL3-RPS12-pro plasmid was transfected into A431-III cells for 24 h, and the cells were treated with DMSO or 10 or 20 µM luteolin for an additional 24 h. The transactivation activities were measured by a luciferase assay. C. The pGL3-RPS12-pro plasmid was transfected into A431-III cells for 24 h, and the cells were treated with 20 or 40 µM quercetin for an additional 24 h. The transactivation activity was measured by a luciferase assay. D. The pGL3-RPS12-pro plasmid was transfected into A431-III cells for 24 h, and the cells were treated with 100 or 200 µM 10058-F4 for an additional 24 h. The transactivation activity was measured by a luciferase assay.

Journal: Journal of Functional Foods

Article Title: RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

doi: 10.1016/j.jff.2015.09.030

Figure Lengend Snippet: Fig. 6 – The transactivation of ribosomal protein S12 (RPS12) is regulated by Akt/mTOR/c-Myc signalling. A. The pGL3-basic plasmid at 0.5 µg, or 0.5 or 1.0 µg of the pGL3-RPS12-pro plasmid was transfected into A431-P cells for 48 h, and the transactivation activities were analysed using a luciferase assay. B. The pGL3-RPS12-pro plasmid was transfected into A431-III cells for 24 h, and the cells were treated with DMSO or 10 or 20 µM luteolin for an additional 24 h. The transactivation activities were measured by a luciferase assay. C. The pGL3-RPS12-pro plasmid was transfected into A431-III cells for 24 h, and the cells were treated with 20 or 40 µM quercetin for an additional 24 h. The transactivation activity was measured by a luciferase assay. D. The pGL3-RPS12-pro plasmid was transfected into A431-III cells for 24 h, and the cells were treated with 100 or 200 µM 10058-F4 for an additional 24 h. The transactivation activity was measured by a luciferase assay.

Article Snippet: Parental A431 cells (human epidermal carcinoma cells called A431-P in this study) were obtained from ATCC (Manassas, VA, USA).

Techniques: Plasmid Preparation, Transfection, Luciferase, Activity Assay

Fig. 7 – The migratory ability of A431-III cells is blocked by Akt/mTOR/c-Myc signalling. A. A wound-healing assay was conducted, and the cells were observed for migration into the wound area. A431-III cells were treated with (a) DMSO; (b) 5 nM, (c) 10 nM rapamycin; (d) 5 µM, (e) 10 µM LY294002; (f) 100 µM, (g) 200 µM 10058-F4 for 24 h. B. The migrating cells were measured and analysed using ImageJ software.

Journal: Journal of Functional Foods

Article Title: RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

doi: 10.1016/j.jff.2015.09.030

Figure Lengend Snippet: Fig. 7 – The migratory ability of A431-III cells is blocked by Akt/mTOR/c-Myc signalling. A. A wound-healing assay was conducted, and the cells were observed for migration into the wound area. A431-III cells were treated with (a) DMSO; (b) 5 nM, (c) 10 nM rapamycin; (d) 5 µM, (e) 10 µM LY294002; (f) 100 µM, (g) 200 µM 10058-F4 for 24 h. B. The migrating cells were measured and analysed using ImageJ software.

Article Snippet: Parental A431 cells (human epidermal carcinoma cells called A431-P in this study) were obtained from ATCC (Manassas, VA, USA).

Techniques: Wound Healing Assay, Migration, Software

Fig. 8 – RPS12 promotes the invasion of cancer cells. (A) Two RPS12 shRNA were transfected into A431-III cells, and protein expression was analysed via western blotting. A trans-well assay was used to analyse the invasion ability of A431-III cells transfected with control shRNA (B), two RPS12 shRNA (C, D) and treated with 0.01% DMSO (E), or 200 µM 10058-F4 (F).

Journal: Journal of Functional Foods

Article Title: RPS12 increases the invasiveness in cervical cancer activated by c-Myc and inhibited by the dietary flavonoids luteolin and quercetin

doi: 10.1016/j.jff.2015.09.030

Figure Lengend Snippet: Fig. 8 – RPS12 promotes the invasion of cancer cells. (A) Two RPS12 shRNA were transfected into A431-III cells, and protein expression was analysed via western blotting. A trans-well assay was used to analyse the invasion ability of A431-III cells transfected with control shRNA (B), two RPS12 shRNA (C, D) and treated with 0.01% DMSO (E), or 200 µM 10058-F4 (F).

Article Snippet: Parental A431 cells (human epidermal carcinoma cells called A431-P in this study) were obtained from ATCC (Manassas, VA, USA).

Techniques: shRNA, Transfection, Expressing, Western Blot, Control